Influence of Mobile Phase Online Mixing Mode and Premixing Mode on Refractive Index Detectors
Objective:This paper studies the impact of baseline fluctuation caused by mixed flow compared with different brands of refractive index detectors,proposes improvement and countermeasures,and provides reference and suggestions for the use of refractive index detectors and supporting liquid phase systems.Methods:Using 70+30%acetonitrile water as mobile phase,glucose was measured with refractive index detectors of different brands,and baseline drift,baseline noise,minimum detection concentration and linear range were obtained to investigate the performance of refractive index detector.Results:Pre mixing can reduce the interference of mobile phase mixing on the refractive index detector,but there will still be periodic pulsation interference,and the interference of mixing mode after using the pump is minimal.Conclusion:refractive index detectors is recommended to use the configuration of multi pump after mixing,with high measurement accuracy.If mixing before using the pump,single parallel infusion pump should be used to reduce interference and improve accuracy.
refractive index detectorspulsating disturbancemultiple pumpmeasurement accuracymobile phase
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