The Kinetic Turbidimetric Assay was used to Determine the Content of Bacterial Endotoxin in Samples
To determinate the concentration of bacterial endotoxin in proficiency testing samples and analyze the matters needing attention in the inspection process.According to the method of ChP 2022.Vol Ⅳ:1143.The kinetic turbidimetric method of photometric determination of bacterial endotoxin.Establish a standard curve of bacterial endotoxin test.The optimal dilution ratio and bacterial endotoxin content were determined by pre-experiment.The optimal dilution ratio was selected to determine the bacterial endotoxin content in the samples for three independent and parallel tests.The linear range of bacterial endotoxin was 0.0625～1.0 EU/mL.The standard curve is LgT=2.8059+(-0.28966LgC)(|r|=0.9982).The endotoxin test wasnt interfered by 1000 times diluted solution.The recovery rate of bacterial endotoxin was 50%～200%.The results of T1,T2 and T3 were 83.80 EU/mL,82.70 EU/mL and 83.00 EU/mL.The kinetic turbidimetric method is reliable,accurate and stable for the proficiency testing samples.
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