Extraction and Application with Gallic Acid of Jussiaea repens L.
Jussiaea repens L.is a commonly used medicinal plant in China.This article optimized the extraction method of gallic acid from Jussiaea repens L.and studied its in vitro antibacterial activity and anti-tumor cell activity.By using the acid extraction method and combining orthogonal experiments,the optimal extraction conditions are:2.5 mol∙L-1 hydrochloric acid,acid extraction at 1:75 and 100℃for 3.5 hours,and the extraction amount of gallic acid from the Jussiaea repens L.is 5.12 mg∙g-1.The extracted gallic acid has significant inhibitory effects on Escherichia coli,Bacillus subtilis and Staphylococcus aureus,with a minimum inhibitory concentration of 51.2 µg∙mL-1.Gallic acid from Jussiaea repens L.also has a certain inhibitory effect on the growth of A549 and MCF-7 tumor cells,with IC50 values of 57.33 µg∙mL-1 and 145.0 µg∙mL-1 respectively.
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