Determination of 3 Isothiazolinone Fungicides in Water-based Adhesives by Ultra-performance Liquid Chromatography-tandem Mass Spectrometry
A method was developed for rapid determination of 2-methyl-4-isothiazolin-3-one(MI),5-chloro-2-methyl-4-isothiazolin-3-one(CMI),1,2-benzylisothiazolin-3-one(BIT)3 isothiazolinone fungicides in water-based adhesives using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The pre-treatment used water as the dispersant and methanol as the extractant,and the target compound was extracted by ultrasound for 40 min.multiple reaction The monitoring(MRM)technology,positive scan mode,multi-channel,time-phased were used to analyse target compounds.3 isotope internal standard method were choiced for Quantitative analysis.The calibration curves of 3 compounds with correlation coefficients were in the range of 0.9991～0.9999,The limits of detection of the method for target compounds ranged from 0.06 to 0.10 mg·kg-1,the limits of quantitation varied from 0.21 to 0.32 mg·kg-1,The recoveries of target compounds ranged from 89.1％ to 103.5％ with relative standard deviations(RSDs,n=7) was less than 3.4％.This method had simple pre-treatment,high recovery rate and good repeatability,and could meet the rapid detection requirements of 3 isothiazolinone fungicides in water-based adhesives.
UPLC-MS/MSisothiazolinonefungicidesMRMinternal standard
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