Study on the Quality Evaluation of Xiaoyanling Capsules based on HPLC Characteristic Chromatogram
Objective:To establish a high performance liquid chromatography method for the characterization of Xiaoyanling capsules,and to provide a new idea for the quality evaluation of Xiaoyanling capsules through similarity evaluation and cluster analysis.Methods:A Waters Xselect C18 column was selected for HPLC,Gradient elution was performed with acetonitrile-0.1%phosphoric acid solution as mobile phase,the flow rate was set to 1.0mL/min,the column temperature was 30℃,and the detection wavelength was selected at 280 nm.Using the glycyrrhizin peak as the S-peak,32 batches of Xiaoyanling capsule characteristic atlas files were imported into the"TCM Chromatographic Fingerprint Similarity Evaluation System"(2012.1 edition)for similarity calculation.SPSS 26.0 software was used for cluster analysis of the results.Results:There were 14 common characteristic peaks in the HPLC chromatogram of 32 batches of Xiaoyanling capsules.Except for 5 batches of samples,the similarity of the other samples was greater than 0.900.The similarity results were basically consistent with the results of cluster analysis.Conclusion:The research method and analysis results can provide a new idea for the identification and quality control of Xiaoyanling capsules.
万方数据
维普
