Study on the Determination of Baicalin in Yinghua Loquat Granules
Objective:to establish a HPLC method for the determination of Baicalin in Yinghua pipa granules and to provide a method for the quality control of the preparation.Methods:the content of Baicalin in the preparation was determined by HPLC,C18 column,and mobile phase methanol-0.1%phosphoric acid solution(47∶53),wavelength 280 nm.Results:the separation degree of Baicalin in the preparation was good,and there was a good linear relationship between the injection volume(0.04635 μg～0.3708 μg)and the peak area,Y=62.727x-0.0286(r2=1),the average recovery was 96.95%,RSD=0.41%(N=6).Conclusion:the method is specific and can be used for the quality control of Baicalin.
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