Construction and Application of Organic Small Molecule Reactive Fluorescent Probe
Fluorescent probe detection has attracted much attention due to its inherent advantages of high sensitivity,high resolution,and real-time detection and imaging in living samples,and has been frequently applied in chemical detection as well as clinical and biological imaging in various fields.Among them,near-infrared small molecule fluorescent probes with low background interference,high sensitivity,and deep tissue penetration can overcome the limitations posed by traditional fluorescent probes,but also show their unique advantages in bioimaging,which is attracting attention from more and more fields.Compared with traditional fluorescent dyes,compounds with Excited-State Intramolecular Proton Transfer(ESIPT)mechanism have a larger Stokes shift,which can effectively eliminate internal filtering and fluorescence self-absorption effect,reduce the interference of its fluorescence,and improve the sensitivity of molecular probe detection.The sensitivity of molecular probe detection can be improved by reducing the interference of fluorescence,and the compounds have strong modifiability,which can optimize the optical properties of the compounds through molecular docking.Therefore,in this paper,we designed and synthesized a near-infrared ESIPT-type organic small molecule fluorescent probe by combining the advantages of near-infrared and ESIPT,and investigated the feasibility of its application in the detection of hydrazine hydrate.
excited state intramolecular proton transferesiptorganic fluorescent probesnear infraredhydrazine hydrate
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