Determination of Active Ingredients in Nanyue Yunwu Tea and its Methodology
Objective:A HPLC method for the determination of theanine,five catechins(+C,EC,EGC,EGCG,ECG),total flavonoids and caffeine in tea was established,and the quality of Nanyue Yunwu tea was evaluated.Methods:According to GB/T 8303-2018,the effective components in tea were extracted and deteminated by Agilent Eclipse XDB-C18 chromatographic column(4.6 mm×278 nm,5 μm),mobile phase was 2％acetic acid water(A)-2％acetic acid acetonitrile(b),flow rate was 0.8 mL·min-1,column temperature was 30 ℃,and detection wavelengths were 203 nm,278 nm,360 nm,respectively.Results:The results of methodology showed that+C,EC,EGC,EGCG,ECG,caffeine,L-theanine and rutin were 50.00 μg·mL-1～150.00 μg·mL-1,50.00 μg·mL-1～150.00 μg·mL-1,100.00 μg·mL-1～200 μg·mL-1 respectively.The linear relationship is good in the concentration ranges of 10.00 μg·mL-1～100.00 μg·mL-1,100.00 μg·mL-1～400.00 μg·mL-1 and 10.00 μg·mL-1～100.00 μg·mL-1,and the recovery rate is 92.49％～105.56％.The results of methodology showed that the linear relationships of+C,EC,EGC,EGCG,ECG,caffeine,L-theanine and rutin were good in the concentration ranges of 50.00～150.00 μg·mL-1,50.00 μg·mL-1～150.00 μg·mL-1,50.00～150.00 μg·mL-1,100.00 μg·mL-1～250 μg·mL-1,100.00 μg·mL-1～300.00 μg·mL-1,10.00 μg·mL-1～100.00 μg·mL-1 and 100.00 μg·mL-1～400.00 μg·mL-1,respectively.And the recovery rate ranged from 92.49％-105.56％.Under the above conditions,the contents of theanine,catechins,total flavonoids and caffeine in Nanyue Yunwu tea were 67.32 mg·g-1,260.42 mg·g-1,3.712 mg·g-1 and 34.05 mg·g-1,respectively.Conclusion:The contents of theanine,catechins and total flavonoids in Nanyue Yunwu tea are relatively high.The method is simple,accurate,rapid and reproducible,and can provide scientific basis for the quality control of tea.
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