Evaluation of Uncertainty in the Determination of Five Drugs including Mupirocin in Cosmetics by HPLC-MS/MS
A mathematical model was established to analyze the sources of uncertainty in the determination of five drugs such as mupirocin in cosmetics by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),including sample weighing and the volume measurement of sample solution,sample measurement repeatability,reference substance weighing and dilution process,standard curve fitting,etc.The uncertainty in the determination result of each component content was evaluated.The results showed that the extended uncertainties of mupirocin,fusidic acid,timolol,terbinafine,and erythromycin were 0.01674％,0.01720％,0.00186％,0.00090％and 0.00092％(k=2)when their concents in cosmetics were 0.3218％,0.3185％,0.0320％,0.0159％and 0.0164％.This evaluation provides a scientific basis for the accuracy of the measurement results of five components in cosmetics,including mupirocin.
万方数据
维普
