摘要
目的:评价白鲜皮不同极性溶剂提取物的抗菌活性.方法:用乙醇、乙酸乙酯、石油醚三种溶剂制备白鲜皮提取物.观察三种提取物对金黄色葡萄球菌抗菌效果,选出抗菌效果最好的提取物,采用二倍稀释法测最小抑菌浓度,用鉴别试剂去鉴别该提取物可能含有的化学成分.结果:三种提取物中抑菌率强弱顺序为石油醚提取物、乙酸乙酯、乙醇提取物,其中乙醇提取物几乎没有抗菌效果.石油醚提取物的最小抑菌浓度为0.125 g/mL.初步判定,石油醚提取物中可能含有生物碱、黄酮、油脂、甾体或三萜类化合物.结论:白鲜皮石油醚提取物对金黄色葡萄球菌有较好的抗菌效果,为进一步从脂溶性成分提取分离抗菌有效成分,提供了理论参考.
Abstract
Objective:To evaluate the antibacterial activity of different polarity solvent extracts of cortex dictamni.Method:Cortex dictamni extracts were prepared with three solvents:ethanol,ethyl acetate,and petroleum ether.The antibacterial effects of the three extracts on Staphylococcus aureus were observed,and the extract with the best antibacterial effect was selected.The minimum inhibitory concentration was measured by the double dilution method,and the possible chemical components in the extract were identified with identification reagents.Results:Among the three extracts,the order of antibacterial rate is petroleum ether extract,ethyl acetate,ethanol extract,with the ethanol extract having almost no antibacterial effect.The minimum inhibitory concentration of the petroleum ether extract is 0.125 g/mL.It is preliminarily determined that the petroleum ether extract may contain alkaloids,flavonoids,fats,sterols,or triterpenes.Conclusion:The petroleum ether extract of cortex dictamni has a good antibacterial effect on Staphylococcus aureus,providing a theoretical reference for further extraction and separation of antibacterial effective components from liposoluble components.
维普
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