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从茶叶中提取茶多酚和咖啡因实验优化研究

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茶叶中富含茶多酚、咖啡因等对人体有益的物质,如何实现其高效提取是当前困扰茶酒产业发展的瓶颈难题.本文以都匀毛尖为研究对象,基于索氏提取仪高效萃取茶多酚和咖啡因,采用单因素实验法研究了萃取剂、料液比、萃取时间、萃取温度对萃取效果的影响规律,结合正交实验法得到了高萃取率的优惠工艺条件.研究结果表明:各因素对萃取率的影响程度依次为:萃取剂(A)>料液比(D)>萃取温度(C)>萃取时间(B),最佳工艺条件为A3B2C2D3,即萃取剂为酱酒,萃取时间为5 h,萃取温度为90℃,料液比为15∶1,茶多酚的萃取率可达35.05%,咖啡因的萃取率可达9.36%.本文研究成果为高品质茶酒制备提供了新的思路.
Optimization of Extraction of Polyphemus and Caffeine from Tea-leaves
Tea was rich in various substances that were beneficial to the human body,such as tea polyphenols,caffeine,etc.How to achieve efficient extraction of beneficial substances was currently a bottleneck problem that hindered the development of the tea-wine industry.This article takes Duyun Maojian as the research object,and efficiently extracted the tea polyphenols and caffeine contained in it using a Soxhlet extractor.The single factor experimental method was used to study the influence of process conditions such as extractant,solid-liquid ratio,extraction time,and extraction temperature on the extraction effect.Combined with orthogonal experimental method,favorable process conditions were obtained.The research results indicated that the degree of influence of various factors on the extraction rate was as follows:extractant(A)>material liquid ratio(D)>extraction temperature(C)>extraction time(B).The optimal process conditions were A3B2C2D3,which meant the extractant was sauce wine,extraction time was 5 hours,extraction temperature was 90℃,and material liquid ratio was 15∶1.Under these conditions,the extraction rate of tea polyphenols was 35%,and the extraction rate of caffeine was 10.8%.The results of this article provided an new idea for the preparation of high-quality tea-wine.

tea polyphenolscaffeinesoxhlet extractorliquid chromatography

郭举、王相勇、李宏河、罗贞标、吴福勇、史天柱

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茅台学院,贵州 仁怀 564500

茶多酚 咖啡因 索氏提取仪 液相色谱

2020年贵州省高等学校教学内容和课程体系改革项目

2020256

2024

广东化工
广东省石油化工研究院

广东化工

影响因子:0.288
ISSN:1007-1865
年,卷(期):2024.51(16)