In order to study the effects of different liquid culture conditions on the growth of Cordyceps cicadae,single factor experiment and orthogonal experiment were carried out with the amount of mycelium growth as the index,carbon source,nitrogen source,inorganic salt and culture temperature as the factors.Under the premise of 150 r/min rotation speed and 5%inoculation amount,orthogonal experiment was used to determine the optimal medium formula:soluble starch 20 g/L,peptone 15 g/L,KH2PO4 1 g/L,and the optimal culture temperature was 28℃.Under these conditions,the mycelial biomass of Cordyceps cicadae was 624.48±6.18 g/L.The optimum culture medium formula and culture temperature were obtained,which provided a theoretical basis for the optimization of liquid culture conditions of Cordyceps cicadae.
维普
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