Simultaneous Determination of 15 Soporific and Sedative Drugs in Blood by UPLC-MS/MS
To establish a method for the simultaneous determination of 15 soporific and sedative drugs in blood by ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS).The sedative drugs in the blood were extracted by a rapid and convenient acetonitrile precipitation protein method,separated by an Acquity BEH C18(2.1 mm×100 mm,1.7 μm)chromatographic column,and detected by multiple reaction monitoring(MRM)mode under electrospray ion source positive ion mode(ESI+).The results showed that the detection limits of 15 hypnotic sedative drugs in blood were between 0.08 ng/mL～0.29 ng/mL,and the quantitative limits were between 0.25 ng/mL～0.96 ng/mL;The linear relationship was good in the range of 1 ng/mL～50 ng/mL,and R2 was greater than 0.9979;In the low,medium and high(2 ng/mL,10 ng/mL,50 ng/mL)concentrations,the matrix effect was between 81.06%～100.72%,the extraction recovery was between 76.86%～102.0%,and the intra-day and inter-day precision RSD were not more than 7.68%.The method has good specificity,high sensitivity and good accuracy,and can be used for rapid detection of 15 soporific and sedative drugs.
bloodsoporific and sedative drugspublic securityultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
万方数据
维普
