Research and Application for Contents Differentiation of Three Ginsenoside Compounds in Ginseng,Red Ginseng,American Ginseng and by UPLC-MS/MS
Objective:To establish an UPLC-MSMS method to detect the contents of three ginsenoside compounds in Ginseng,Red Ginseng,American Ginseng.Methods:Waters ACQUITY BEH C18 column(2.1 mm×100 mm,1.7 μm)was used as the chromatographic column,0.1%formic acid and tetrahydrofuran-acetonitrile(5∶95)was the mobile phasein gradient elution,and the flow rate was 0.3 mL/min.The Multiple reactions monitoring(MRM)mode was used as the detection mode,with electro-spray ionization(ESI)source.Results:The linear relationships of pseudoginsenoside F11,ginsenoside Rf,Re were good between 0.005μg/mL～0.20 μg/mL,0.01μg/mL～0.40 μg/mL,0.01 μg/mL～0.40 μg/mL;the average recoveries were 93.9%(RSD=1.9%),91.9%(RSD=2.3%)and 101.3%(RSD=4.1%),the limits of detections(LODS)were 0.0005 μg/mL,0.001 μg/mL,0.003 μg/mL.The limits of quantitation(LOQS)were 0.0017 μg/mL,0.003 μg/mL,0.010 μg/mL.Conculsion:The method is accurate,rapid,sensitive and convenient,can be used for quantitative determination of three ginsenoside compounds in Ginseng,Red Ginseng,American Ginseng.
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