基于网络药理学方法探究灯盏花素联用三七总皂苷治疗脑梗死(cerebral infarction,CI)的潜在作用机制.运用PubChem以及Swiss Target Prediction数据库获取灯盏花素(breviscapine)和三七总皂苷(panax notoginseng saponins,PNS)的主要活性成分以及相关靶点.利用GeneCards、DisGeNET、Drugbank和PharmGkb数据库收集的相关疾病靶点,与成分靶点取交集绘制维恩图.在STRING平台获取蛋白-蛋白相互作用(protein-protein interaction,PPI)网络,采用Cytoscape3.7.2软件CytoNCA插件构建网络图并进行可视化处理.采用DAVID数据库进行基因本体论(Gene Ontology,GO)生物过程和京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析.有效成分6种,共检测到 42个有效成分靶点,CI相关靶点 960个,取交集后得到灯盏花素和三七总皂苷治疗CI的潜在靶点15个.对交集靶点富集,GO富集分析共涉及生物过程111个,细胞组分19个,分子功能20个,KEGG通路富集得到 55条通路,结合蛋白-蛋白相互作用网络分析与富集分析最终得到TNF、AKT1、EGFR、PTGS2、STAT3 共5个核心靶点.本研究初步探索了灯盏花素联用三七总皂苷治疗脑梗死的作用机制为后续研究提供基础.
A Network Pharmacology-based Investigation of the Potential Mechanism of Action of Breviscapine in Combination with Panax Notoginseng Saponins in the Treatment of Cerebral Infarction
To investigate the potential mechanism of action of breviscapine in combination with panax notoginseng saponins(PNS)in the treatment of cerebral infarction(CI)based on a network pharmacology approach.We used PubChem and Swiss Target Prediction databases to obtain the main active ingredients and related targets of breviscapine and panax notoginseng saponins.Using the relevant disease targets collected from GeneCards,DisGeNET,Drugbank and PharmGkb databases,intersections were taken with the component targets to draw venn diagrams.Protein-protein interaction(PPI)networks were obtained from STRING platform,and CytoNCA plug-in of Cytoscape 3.7.2 software was used to construct network diagrams and visualise them.Gene Ontology(GO)biological process and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed using DAVID database.There were 6 active ingredients,42 active ingredient targets were detected,960 CI-related targets,and 15 potential targets for CI treatment of breviscapine and panax notoginseng saponins were obtained after taking the intersection.For the intersection target enrichment,GO enrichment analysis involved 111 biological processes,19 cellular components,20 molecular functions,KEGG pathway enrichment obtained 55 pathways,combined with protein-protein interaction network analysis and enrichment analysis finally obtained a total of 5 core targets of TNF,AKT1,EGFR,PTGS2,STAT3.This study initially explored the mechanism of action of breviscapine combined with PNS in the treatment of cerebral infarction,and provided a basis for subsequent studies.
万方数据
维普
