By studying the pre-treatment methods of vanilla,it was found that enzymatic hydrolysis was used to increase the extraction efficiency of effective ingredients.The optimal enzymatic hydrolysis process was as follows:the applicable amount of cellulase was 0.4%,the applicable amount of pectinase was 0.1%,the ratio of vanilla to enzymatic hydrolysis solution was 1∶,the pH was 5,the temperature was 45℃,and the enzymatic hydrolysis time was 180 minutes.By analyzing different extraction processes of vanilla,the heating reflux method was selected for research.Through orthogonal experiment,the extraction effect of vanillin in vanilla under different extraction conditions was determined.Determine the extraction temperature was70℃,the ethanol concentration was 70%,the reaction time was 6 hours(3∶1.5∶1.5),and the solid-liquid ratio was 1∶8.The effective components of vanilla extract were determined by GC-MS analysis.Through cigarette smoking evaluation,the recommended dosage in cigarettes was determined to be 0.005%～0.02%.The application of vanilla extract in cigarettes was beneficial to improve the flavor characteristics of cigarettes,make the cigarette smoke sweet and mild,and have comfortable bean,milk and mellow aromas.
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