Optimization of Fermentation Medium for Municipal Sludge Composting Strain Bacillus stercoris HDF-03
The objective of this experiment was to enhance the biomass production of Bacillus stercoris HDF-03,a strain derived from municipal sludge compost.The effects of different components in the fermentation medium on the biomass yieldwere investigated.Plackett-Burman experiment revealed that fish meal and soybean meal were the most significant factors for the increase of biomass yield,and their optimal concentration ranges were determined through a steepest climb experiment.Subsequently,these factors were optimized using the response surface method,and the results showed that optimal composition of the fermentation medium wasfish meal(18.75 g∙L-1),soybean meal(47.50 g∙L-1),magnesium sulfate heptahydrate(0.55 g∙L-1),potassium dihydrogen phosphate(1.5 g∙L-1),and dipotassium hydrogen phosphate(3.0 g∙L-1).The dry weight of Strain HDF-03 cells cultivated under the optimum condition achieved 3.737 g∙L-1,which was 13.21%higher than the biomass yield of the initial medium.
municipal sludgefunctional bacteriaresponse surface methodologyPlackett-Burmanfermentation optimization
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