Analysis of Characteristic Fragment Structures of Sodium-adducted Sucralose in ESI Positive Mode[M+Na]+
To analyze the fragmentation processes of sucralose in the ESI positive mode[M+Na]+,and obtained the fragmentation pathway and the product molecule,so as to provide a reference for establishing a rapid screening database based on characteristic fragment structure.By optimizing the program and adjusting the mobile phase,the spectral patterns of the precursor ions of sucralose with sodium were analyzed,and the collision energy,intensity and neutral loss of the characteristic product ions was analyzed,the characteristic fragments were identified,and the potential fragmentation pathways were inferred.Finally,The validity of the proposed fragmentation pathways and molecular formulas were verified through calculations based on the abundance ratios of chlorine isotopes in nature.The main ion in the Q1 mass spectrum was[M+Na]+with the addition of sodium ions,In the Q2 mass spectrum,two major product ions,239 m/z and 221 m/z,as well as two minor ions,was observed,It was two major ions exhibited high relative abundance and good stability.The rationality of the proposed fragmentation pathways and molecular formulas was confirmed by calculating the abundance ratios of chlorine isotopes in nature.Conclusion This study provides a reference for the application of sucralose in ESI positive mode and establishes a theoretical foundation for the analysis of sucralose mass spectral fragmentation patterns.
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