Study on the Barrier Repair Effect of Aloe vera Gel
This study is to dissect the effect and mechanism of Aloe vera gel on barrier repair.The effects of Aloe vera on the cell proliferation and cell migration rate of human immortalized keratinocytes and dermal fibroblasts were studied by in vitro cell experiments.The cell model of UVA radiation injury was used to detect the ROS content,skin barrier related gene expression level and type Ⅰ collagen secretion.Human clinical test was used for evaluation based on two dimensions:instrumental measurement and subject self-evaluation.The results showed that adding 1 mg/mL～4 mg/mL Aloe vera gel promoted the cell proliferation and migration of keratinocytes and dermal fibroblasts(P<0.01),significantly improved the survival rate of cells damaged by ultraviolet rays,and at the same time reduced the intracellular ROS content(P<0.01).Higher concentrations of Aloe vera gel significantly increased the expression levels of FLG and Cldn-1 genes,and promoted the secretion of type Ⅰ collagen(P<0.01).After using Aloe vera gel for 28 days,the subjects skin redness,scaling,dryness,sensitivity,burning sensation and tightness were all improved compared to before use.After using the product for 4 to 6 days,the water content of the stratum corneum increased significantly,and TEWL value,skin hemoglobin,and red area significantly decreased(P<0.05).In conclusion,Aloe vera gel has barrier repair effects.This effect may be achieved by promoting epidermal cell proliferation and cell healing,inhibiting the production of ROS,promoting barrier gene expression and collagen secretion,thereby reduci ng epidermal cell damage and apoptosis.
Aloe vera gelskin barrierrepairkeratinocytesfibroblasthuman clinical test
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