Comparative Study on The Related Substance of Distearoyl Phosphatidylcholine by HPLC-ELSD and TLC Scanning
Objective:To establish a method of related substance in Distearoyl Phosphatidylcholine.Methods:Compared between TLC scanning and HPLC-ELSD.The TLC separation was achieved using a silica gel G60 plate,trichloromethane-methanol-water-ammonia(65∶25∶4∶0.4)as the developing agent,detection wavelength was 450 nm.The HPLC separation was achieved using a C8 column,the mobile phase was composed of methanol and water with gradient elution,the detector was evaporative light-scattering detector.The logarithm of the concentration was the x-axis,The logarithm of area was the y-axis,performed linear regression calculations.Then verified the established method by methodology.Results:Only a small amount of known impurities were detected by TLC scanning,the unknown impurity couldn't effectively detected.In the aspect of sample amount,the detection of impurity and operation convinence,HPLC-ELSD was superior to TLC scanning.Conclusion:The new HPLC-ELSD method with high sensitivity and accurate determination overcomes the shortcoming of the original method which cannot be accurately quantified,can be used as quality control method of distearoyl phosphatidylcholine.
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