Study on the Screening Method for Anti-tumor Active Components of Phellodendron Amurense Rupr
Objective:To develop a new screening method for the active ingredients of TCM.Method:On the basis of normal cell metabolism,the supernatant of tumor cells in different time periods was microextracted by dispersed liquid phase,and the active component groups were effectively detected,and the potential active ingredient groups were found according to the chromatographic peak changes.Breast cancer cells were used as the platform for active screening.In the natural absorption state of cells,the active components were screened and captured,and then HPLC analysis.Results:The anti-tumor active components in the yellow cypress were successfully screened as berberine and Bamatin.Conclusion:This study attempts to provide a convenient,universal and efficient new method for the screening of active ingredients and the comprehensive evaluation of TCM quality,and to provide a theoretical and experimental basis for the basic research of TCM medicinal substances.
yellow cypresstraditional Chinese medicinescreeningactive ingredients
万方数据
维普
