Effect of Different Prescribed mRNA-LNP on Cell Expression in Vitro
Nucleic acid drug delivery systems are technologies that facilitate the efficient delivery of nucleic acid drugs into cells,allowing them to exert their effects.Despite the high bioactivity and specificity of nucleic acid drugs,their applications still face various challenges.Therefore,research on the formulations of nucleic acid delivery systems is crucial.To investigate the impact of different formulations of lipid nanoparticles(LNPs)on in vitro expression in cells,we used GFP-mRNA as a test gene to prepare LNPs with different formulations and explore their mechanisms of action.In this study,we prepared various LNP formulations and chose 293T cells with high expression as the transfection cells.The fluorescence intensity was detected using a fluorescence inverted microscope to analyze and compare the transfection efficiency.We found that a high proportion of empty LNPs affects the in vitro transfection efficiency of cells,laying a foundation for the development of high-quality mRNA drug formulations.
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