Preparation of MAdCAM Antibody-conjugated Liposomes and Colonic Inflammatory Tissue Binding Studies
In this study,an immunoliposome targeting MAdCAM-1(Mucosal addressin cell adhesion molecule-1)was prepared,and its specific binding ability was verified by in vitro tissue IHC experiments,so as to provide a new solution for the treatment of enteritis.In this paper,MECA-367 protein(MadCAM protein antibody)was first introduced by SATA for sulfhydryl group introduction,then conjugated to DSPE-PEG-2K-MAL,and then loaded on liposomes.Immunoliposomes targeting MAdCAM were prep ared,and SDS-PAGE,HPLC and particle size were used as evaluation indexes.the DSS animal experimental model was established and IHC experiments were performed.The SDS-PAGE bands were successfully conjugated,and the results of preparation characterization showed that the average particle size of immunoliposomes was 87 nm,and the particle size distribution was uniform(polydispersity index,PDI≤0.1),the loading efficiency was 100%verified by HPLC results,the affinity was good by BLI,and the binding capacity was verified by positive IHC results.Conclusion:In this paper,MAdCAM antibody-conjugated liposomes were successfully prepared,and its specific binding to colonic inflammatory tissues was successfully verified in vitro experiments.This preparation process method is proved to be feasible and can be continuously explored.
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