Cell experiment,hyaluronidase experiment and human clinical experiment were used to investigate the soothing and repairing effects of Centella asiatica extract.Cell experiments were conducted to study the effects of Centella asiatica extract on proliferation of RAW264.7 cells(mouse macrophages)and secretion of IL-6 in RAW cells,and the effects of Centella asiatica extract on migration of HaCaT cells(keratinocytes).Hyaluronidase assay was used to evaluate the soothing effect of Centella asiatica extract by inhibiting the activity of hyaluronidase.The human clinical experiment measured the skin water loss TEWL value and skin water content MMV value of volunteers before and after using Centella asiatica extract,and used VC20 image system to detect fine line improvement.The results showed that when the volume concentration of Centella asiatica extract was 0.003%and 0.0015%,the proliferation of RAW cells and IL-6 concentration were significantly inhibited.The activity of hyaluronidase was significantly inhibited by 0.2%,0.1%and 0.05%of the extracts.The extract of 100 μg/mL could significantly promote the migration of HaCaT cells.Human clinical experiments showed that the change rate of skin transdermal water loss TEWL value was-37.86%(P＜0.001),the change rate of skin water content MMV value was 65.09%(P＜0.001).To sum up,Centella asiatica extract has certain soothing and repairing effects.
关键词
积雪草/舒缓与修护/透明质酸酶/细胞实验/人体临床实验
Key words
centella asiatica/sooth and repair/hyaluronidase/cell experiment/human clinical trial
维普
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