Determination of six Estrogens in Beverages by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
Objective:To establish an efficient and rapid ultra-high performance liquid chromatography tandem mass spectrometry method for the detection of estradiol,estradiol,estrone,dienestrol,diethylstilbestrol and hexestrol in beverages.Methods:Three representative beverage substrates were selected and extracted with acetonitrile and QuEChERS extraction kit two times,combined with the extraction solution,nitrogen was blown to dry at 50℃,and the residue was dissolved with methanol to 0.5%ammonia water(volume ratio 9∶1).Methanol and 0.5%ammonia solution were separated by C18 column.Qualitative and quantitative analysis were carried out by LC-MS/MS multi-reaction monitoring mode.Quantitative analysis was carried out by external standard method.Result:The results showed that the detection limit of the method was 0.3 μg/kg～3.0 μg/kg.The average recovery was 72.5%to 119%and the relative standard deviation was 6.7%to 11.4%in the range of 10 μg/kg～100 μg/kg.The method has high sensitivity,fast and efficient pretreatment,and is suitable for the detection and quantitative analysis of 6 estrogen in beverages.
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