Determination of Artemisia Argyi Volatile Oil,Eucalyptol and Borneol in Different Periods in Lichuan County
Objective:The content of volatile oil in artemisia argyi of Lichuan county during different period was determined,and the contents of the index components of eucalyptol and borneol were compared,which offered the basis for quality control and comprehensive exploitation of artemisia argyi.Methods:The volatile oil of artemsia argyi in different harvesting periods from May to October was extracted by method A of the 20 edition of the pharmacopoeia.GC8890 gas chromatography analyzer was used to program temperature rise.The carrier gas was five 9 high-purity N2,split sampling,and the flow ratio was 10∶1.Results:The volatile oil content of artemisia argyi in Lichuan country was different in each period,the highest was 1.26%(June),the lowest was 0.71%(May);All volatile oil samples contained eucalyptol and borneol,The concentrations of eucalyptol and borneol were in the range of 0.1 mg·mL-1~1.4 mg·mL-1 and 0.01-0.3 mg·mL-1,respectively,and had a good linear relationship with the corresponding measured values,and the correlation coefficient r was above 0.999.The average recoveries and relative standard deviations(n=6)of eucalyptol and borneol were 93.00%,98.30%and 1.67%,1.15%.The maximum contents of eucalyptol and borneol in June were 1.5 mg·g-1 and 0.7 mg·g-1,and the minimum contents were 0.8 mg·g-1 and 0.2 mg·g-1 in October.Conclusion:The content of volatile oil,eucalyptol and borneol in artemisia argyi is the maximum in June,which is the best time to harvest artemisia argyi.
different periodsartemisia argyigas chromatographyeucalyptolborneolcontent determination