Transformation of Ginsenoside Rd from Ginsenoside Rb1 Catalyzed by Food-grade Commercial Enzymes
The enzyme with the best activity for transforming ginsenoside Rb1 into Rd was screened out from 11 food-grade commercial enzymes from different manufacturers,and the effects of reaction time,enzyme amount,initial pH,and temperature on the conversion efficiency were investigated by single factor experiment.The results showed that the β-glucanase(PangBo)was the most suitable invertase.Under the optimum conditions:2 h of reaction time,0.3 mg/1.5 mg Rb1 of enzyme amount,5.5 of pH,and 55℃of temperature,the biotransformation efficiency of Rd was 98.01%±1.19%(n=3).The use of β-glucanase(PangBo)is an efficient,green,and safe enzyme conversion method for the preparation of ginsenoside Rd.
万方数据
维普
