The Extraction of Lactobacillus Lysate and its Application in Cosmetics
This study used probiotic lactic acid bacteria as the fermentation strain and polysaccharide content as the indicator to investigate key parameters such as seed liquid cultivation time,inoculation amount,pH regulation,cultivation time,ultrasonic power for bacterial fragmentation,and ultrasonic time,in order to determine the optimal fermentation extraction process.Lactic acid bacteria polysaccharides were prepared by enzymatic hydrolysis,heating to remove macromolecular peptides,ethanol precipitation,and in vitro efficacy testing,including ABTS experiment,DPPH,tyrosinase inhibition experiment,and moisturizing experiment.Prepare raw materials extracted through fermentation into products of different dosage forms to explore the application of raw materials in different dosage forms,and evaluate the stability,safety,and efficacy of the products to determine the optimal concentration and application range in cosmetic formulas.