Optimization of Anaerobic Bacteria for Nitrogen Removal from Landfill Leachate Using Anaerobic Sludge to Produce Methane as A Carbon Source
As a typical kind of wastewater containing high ammonia nitrogen and organic matter,it has strict requirements for total nitrogen concentration emissions of landfill leachate from garbage.However,traditional heterotrophic denitrification requires additional organic carbon and this method can lead to secondary pollution and much more CO2 emissions.Denitrifying anaerobic methane oxidation(DAMO)is a process that utilizes methane(CH4)as an electron donor and carbon source for denitrification.Moreover,anaerobic digestion can use organic matter in sludge as a fermentation substrate to produce CH4 under anaerobic conditions.At the same time,combining DAMO with anaerobic ammonia oxidation(Anammox)to achieve deep and complete denitrification of landfill leachate is an economical solution.Based on this background,this work focuses on the selection of several anaerobic bacteria on the market for sludge methane production,in order to provide suitable anaerobic bacteria for subsequent deep denitrification experiments.After comparison,the CJW388 class of advanced anaerobic bacteria from Biliqing is selected as a representative of commercially anaerobic bacterial agents.The anaerobic bacteria are used as an additive to investigate the effects of different substrate types,substrate concentrations,and inoculation ratios.The experimental results show that under high concentrations of COD(5000 mg/L),the addition of commercially microbial agents can result in a similar increase and proportion in methane production from sodium acetate and sodium propionate substrates;In the case of low concentration COD(1500 mg/L)feed,the addition of microbial agents can significantly increase the amount and proportion of methane production in sodium acetate substrate compared to sodium propionate substrate.
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