Study on Quality Standard of Hedyotis chrysotricha
Objective:To establish the quality standard for Hedyotis chrysotricha.Methods:Microscopy and thin-layer chromatography were used to identify the characteristics of Hedyotis chrysotricha.The contents of water,total ash,acid-insoluble ash and ethanol soluble extracts were determined to accord to the method of Chinese Pharmacopoeia.HPLC was used for the content determination of ursolic acid and oleanolic acid.The determination was performed on Agilent ZORBAX Eclipse XDB-C18 column,mobile phase:methanol-0.2%acetic acid solution(88∶12);flow rate:0.8 mL/min.The detection wavelength was set at 210 nm and the temperature was 30℃.Results:Microscopic characteristics are obvious;TLC had clear spots,good resolution and strong specificity.Ursolic acid and oleanolic acid showed good linear relationships within the range of 0.0376 mg/mL～0.9399 mg/mL and 0.0074 mg/mL～0.1845 mg/mL,whose average recoveries were 98.61%and 97.63%with the RSDs of 1.07%and 1.13%,respectively.Total ash of medicinal materials was 11.4%～21.4%,acid insoluble ash was 3.0%～11.9%,water content was 8.4%～11.0%,ursolic acid and oleanolic acid was 1.43～3.41 mg/g.Conclusion:This reasonable and feasible method can be used for the quality control of Hedyotis chrysotricha.
万方数据
维普
