Effects of Cobalt Chloride on the Expression of Notch Signaling Pathway in Brain Microvascular Endothelial Cells
Objective:Exploring the effect of CoCl2 induced hypoxic microenvironment on the expression of Notch signaling pathway proteins in cerebral vascular endothelial cells and its possible mechanisms.Methods:Brain microvascular endothelial cells(MECs)were used in this study,and the hypoxic microenvironment in vitro were stimulated.Different concentration of CoCl2(0 μmol/L,10 μmol/L,50 μmol/L,100 μmol/L,200 μmol/L,300 μmol/L,400 μmol/L)were used in MEC cells to establish hypoxia model.CCK-8 cellular viability analysis,flow cytometric cell apoptosis analysis,and cell migration were used to study the effect of CoCl2 on cell migration and proliferation ability.The expression of HIF-1α protein and Notch signaling pathway related proteins were detected by Western blot.Results:With increasing CoCl2 concentrations,MEC cell viability decreased,the ratios of apoptotic cells increased,and MEC cell migration ability decreased.The HIF-1α protein expression and the expression of Notch signaling pathway related proteins peaked at the concentration of 200 μmol/L.Conclusion:CoCl2 induced hypoxia damage can upregulate the HIF-1α and Notch signaling pathway related proteins expression levels.
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