反相高效液相色谱法测定彩妆新型防腐剂BIT的研究
Study on the Determination of BIT in a New Cosmetic Antiseptic by Reverse Phase High Performance Liquid Chromatography
谭丙文 1蔡志勇 1黄紫军 1肖雄1
作者信息
- 1. 广东碧茜生物科技有限公司,广东 中山 528451
- 折叠
摘要
本文研究并建立采用反相高效液相色谱法测定彩妆行业新型防腐剂:1,2-苯并异噻唑啉-3-酮(BIT)的方法.BIT采用色谱纯甲醇常温超声萃取,色谱分离柱为SHIMADZU Shim-pack GIST C18分析柱(4.6 mm*250 mm*5 μm),以色谱纯甲醇∶水=70%∶30%为流动相等度洗脱测定.紫外检测波长λ1=225 nm(定量波长)/λ2=319 nm(辅助定性波长),CTO柱温箱温度设置为30℃.标准曲线线性范围 0.5 μg/mL～100.0 μg/mL,线性方程A=13323.8C-3505.69,相关系数R=0.9998550,最低检出限 0.36 μg/mL,平均回收率 99.3%,相对标准偏差RSD%为0.19%(n=10).该方法简单、灵敏、准确、快速,为我司彩妆类多款产品新型防腐剂BIT的检测及质量控制提供了一个实用便捷的分析方法.
Abstract
This article studies and establishes a method for determining a new antiseptic in the cosmetics industry,1,2-benzisothiazolin-3-one(BIT),using reverse phase high performance liquid chromatography(RP-HPLC-DAD/UVD).BIT adopts chromatographic pure methanol room temperature ultrasonic extraction,and the chromatographic separation column is SHIMADZU Shim pack GIST C18 analytical column(4.6 mm*250 mm*5 μ m).The determination is carried out using chromatographic pure methanol∶water=70%∶30%as the mobile phase elution.The UV detection wavelength is λ1=225 nm(quantitative wavelength)/λ2=319 nm(auxiliary qualitative wavelength),and the temperature of the CTO column temperature box is set to 30℃.The linear range of the standard curve is 0.5 μg/mL to 100.0 μg/mL,with a linear equation A=13323.8C-3505.69,a correlation coefficient R=0.9998550,a minimum detection limit of 0.36 μg/mL,an average recovery rate of 99.3%,and a relative standard deviation RSD%of 0.19%(n=10).This method is simple,sensitive,accurate,and fast,providing a practical and convenient analytical method for the detection and quality control of new antiseptic BIT in our cosmetics products.
关键词
反相高效液相色谱法/等度洗脱/1,2-苯并异噻唑啉-3-酮/超声萃取Key words
RP-HPLC/isocratic elution/1,2-benzisothiazolin-3-one/ultrasonic extraction引用本文复制引用
出版年
2024
NETL
NSTL
万方数据