The Application of the Integrated Exploratory Teaching Mode in The Course of Food Biochemistry Experiments:The Inhibition Kinetics of Brewer's Grains Polyphenols on Amylase
In this paper,the experiment of"inhibition kinetics of brewer's grains polyphenols on amylase"was taken as an example to discuss the application of the integrated exploratory teaching mode in the laboratory course of food biochemistry.Enzyme inhibition is a key area of biochemical research and is essential for understanding complex biochemical processes in living organisms.In this study,the half inhibitory concentration of brewer's grains polyphenols against α-amylase and its inhibition kinetics were determined,aiming to quantify the inhibitory effect of brewer's grains polyphenols and reveal their inhibition mechanism.During the experiment,students not only learned the basic principles and experimental techniques of enzyme inhibition,but also mastered the use of scientific instruments such as ultraviolet-visible spectrophotometers.Through this experiment,the students'experimental operation ability,scientific inquiry ability and teamwork ability have been significantly improved.This experiment promoted interdisciplinary and teaching reform,and provided new ideas and methods for talent training in the field of food biochemistry.
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