肌底液中酵母溶胞产物和多肽组合物的作用研究
A Study on the Efficacy of a Combination of Yeast Lysate Products and Peptide in Serum
钟丽玉 1林玉珊 1闫永涛 1黄楚雯 1吴绮霞 1贺晓静1
作者信息
- 1. 完美(广东)日用品有限公司,广东 中山 528400;广东完美生命健康科技研究院有限公司,广东 中山 528400
- 折叠
摘要
本研究通过复配特定含量的二裂酵母发酵产物溶胞物、棕榈酰三肽-1 和棕榈酰四肽-7 制备出一款具有多功能皮肤功效的肌底液.通过构建小鼠巨噬细胞炎症模型、HaCaT人永生化表皮细胞划痕实验、斑马鱼 eln1、eln2 基因相对表达量测试和面部抗皱功效人体评价实验,评估肌底液的皮肤功效.抗炎功效实验结果表明,0.1％～0.3％浓度的肌底液可以显著减少IL-6 炎症因子的释放,表现出舒缓抗炎功效;细胞体外划痕修复实验结果表明,48 h作用时间内,0.2％浓度的肌底液可以促进人上表皮细胞迁移,显现出皮肤屏障修复功效;斑马鱼eln1、eln2 基因相对表达量测试数据显示 0.25％以上浓度肌底液可显著增加弹性蛋白基因相对表达量,表现出紧致功效.采用Primos CR仪器科学评估使用肌底液8 周的面部抗皱功效人体评价数据,发现该肌底液可以显著改善法令纹细纹和眼下细纹.
Abstract
In this study,a serum with multifunctional skin efficacy was prepared by compounding specific levels of bifida ferment lysate,palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7.The skin efficacy of the serum was evaluated by constructing a mouse macrophage inflammation model,HaCaT human immortalized epidermal cell scratch experiment,zebrafish eln1,eln2 gene relative expression test and facial anti-wrinkle efficacy human evaluation experiment.The results of the anti-inflammatory efficacy experiment showed that 0.1％～0.3％concentration of the serum can significantly reduce the release of IL-6 inflammatory factors,showing soothing and anti-inflammatory efficacy;the results of the in vitro cell scratch assessments showed that,0.2％concentration of the serum can promote the migration of the human epidermal cells within 48 h,revealing the efficacy of the skin barrier repair;zebrafish eln1,eln2 gene relative expression test data show that 0.25％or more concentration of the serum can significantly increase the relative expression of elastin genes,showing firming effect.An 8-week human evaluation data of the anti-wrinkle efficacy of the serum was scientifically assessed using the Primos CR instrument,and it was found that the serum could significantly improve the appearance of fine lines and under-eye wrinkles.
关键词
二裂酵母发酵产物溶胞物/生物活性肽/化妆品功效Key words
bifida ferment lysate/bioactive peptide/cosmetic efficacy引用本文复制引用
出版年
2024
NETL
NSTL
万方数据