Determination of the Differential Compounds in Astragali Radix from Different Origins by High Performance Liquid Chromatography-Mass Spectrometry
To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the simultaneous qualitative and quantitative analysis of 10 chemical constituents of Astragali Radix from different origins.The target analytes were separated on an Agilent Poroshell 120 EC-C18 column(2.1 mm×150 mm,2.7 μm)using 0.1％formic acid acetonitrile and 0.1％formic acid water solution as mobile phase at a flow rate of 0.3 mL/min.Mass spectrometry acquisition was performed using multiple reaction monitoring(MRM)in both positive and negative ion modes.As a result,there existed good linearities for the 10 chemical constituents in the certain concentration ranges(r>0.9970).The mean recoveries were all in the range of 94.6％～106.2％,and the relative standard deviations(RSD)were less than 5％.5 batches(24 batches in total)of Astragali Radix samples did not comply with the content regulations.Through the determination of Astragali Radix samples,the highest content of total saponins was found in ShanXi samples,the highest content of total flavonoids was found in JiLin's.By analysing the ratio of saponins/flavonoids,it has been shown that the origin influences the content of total saponins and total flavonoids in Astragali Radix.
NETL
NSTL
万方数据
