Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution

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外文摘要：Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74 μm in fluorescent beads imaging.The versatility of this lens was further demon-strated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advanc-ing multi-wavelength imaging of large fields of view at high resolution.

外文关键词：

mesoscopic objective lenslarge field-of-viewhigh resolutionmulti-wavelengthwide-field microscopyconfocal laser scanning microscopy

作者：

Xin Xu、Qin Luo、Jixiang Wang、Yahui Song、Hong Ye、Xin Zhang、Yi He、Minxuan Sun、Ruobing Zhang、Guohua Shi

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作者单位：

School of Biomedical Engineering(Suzhou),Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230026,China

Jiangsu Key Laboratory of Medical Optics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Science,Suzhou 215163,China

基金：

National Key R&D Program of ChinaChinese Academy of Sciences Project for Young Scientists in Basic Research

项目编号：

2022YFC2404201YSBR067

出版年：

2024

DOI：

10.29026/oea.2024.230212

光电进展(英文版)

ISSN：

年,卷(期)：2024.7(6)