Stimulated Raman scattering microscopy with phase-controlled light focusing and aberration correction for rapid and label-free,volumetric deep tissue imaging

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外文摘要：We report a novel stimulated Raman scattering(SRS)microscopy technique featuring phase-controlled light focusing and aberration corrections for rapid,deep tissue 3D chemical imaging with subcellular resolution.To accomplish phase-controlled SRS(PC-SRS),we utilize a single spatial light modulator to electronically tune the axial positioning of both the shortened-length Bessel pump and the focused Gaussian Stokes beams,enabling z-scanning-free optical sectioning in the sample.By incorporating Zernike polynomials into the phase patterns,we simultaneously correct the system aberra-tions at two separate wavelengths(～240 nm difference),achieving a～3-fold enhancement in signal-to-noise ratio over the uncorrected imaging system.PC-SRS provides＞2-fold improvement in imaging depth in various samples(e.g.,polystyrene bead phantoms,porcine brain tissue)as well as achieves SRS 3D imaging speed of～13 Hz per volume for real-time monitoring of Brownian motion of polymer beads in water,superior to conventional point-scanning SRS 3D imaging.We further utilize PC-SRS to observe the metabolic activities of the entire tumor liver in living zebrafish in cell-silent region,unraveling the upregulated metabolism in liver tumor compared to normal liver.This work shows that PC-SRS provides unprecedented insights into morpho-chemistry,metabolic and dynamic functioning of live cells and tissue in real-time at the subcellular level.

外文关键词：

SRS 3D imagingphase-controlled light focusingimage aberration correctionsdeep tissue imaging

作者：

Weiqi Wang、Zhiwei Huang

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作者单位：

Optical Bioimaging Laboratory,Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore 117576

基金：

Academic Research Fund(AcRF)from the Ministry of Education(MOE)Academic Research Fund(AcRF)from the Ministry of Education(MOE)Academic Research Fund(AcRF)from the Ministry of Education(MOE)Academic Research Fund(AcRF)from the Ministry of Education(MOE)2024 Tsinghua-NUS Joint Research Initiative FundNational Medical Research Council(NMRC) SingaporeNational Medical Research Council(NMRC) Singapore

项目编号：

Tier 2A-8000117-01-00Tier 1R397-000-334-114R397-000-371-114R397-000-378-114A-0009502-01-00A-8001143-00-00

出版年：

2024

DOI：

10.29026/oea.2024.240064

光电进展(英文版)

ISSN：

年,卷(期)：2024.7(9)