Study on Tissue Culture Breeding Technology of Broussonetia papyrifera
The tissue culture technology of Broussonetia papyrifera was studied by explants disinfection,lateral bud induction,subculture multiplication and root induction.Explants were sterilized using 75％ ethanol and 0.15％ mercuric chloride,the survival rate was 40％.The lateral buds planted in MS medium containg 6-BA 2.0 mg/L and NAA 0.5 mg/L showed the highest bud differentiation rate (80％).The optimum medium for subculture was MS + 6-BA 1.0 mg/L + NAA 0.5 mg/L,the proliferation times was 3.96.The rooting rate of 100％ could be obtained by using 1/2MS + IBA1.0 mg/L or GGR6 0.5 ～ 1.0 mg/L.The results of this study fomed a high potential of large scale commercial production of B.papyrifera.
NETL
NSTL
万方数据
维普
