Effects of Colchicine Treatment on the Seed Germination and Seedling Growth of Bauhinia variegate
The mature seeds of Bauhinia variegate were studied by treating with 0.5, 1.0, 1.5 and 2.0 mg/mL colchicines solution for 12, 24, 36 and 48 h respectively, and the seeds soaked with distilled water were taken as control. The germination rate and the mutant seedling growth were observed after the treatments. The results showed that with the increase of colchicines concentration, seed germination rate decreased. 2.0 mg/mL colchicines solution caused death of the seeds, and 0.5,1.0 mg/mL colchicines solution treatment for 24 h was the most beneficial to the germination of the seeds. With the increase of colchicines solution concentration, the radicle elongation was inhibited in different degrees; colchicine could cause the inhibition effect on continue development of germinating seed embryo. The change of seedling ground diameter was consistent with that of the seedling height after 7 months later, it showed that after soaking the seeds for 36 h in colchicine solution (1.0 mg/mL), the seedling ground diameter and height were both the highest. High concentration(2.0 mg/mL)colchicine solution on average leaf split angle played an important role in germination promoting. The stimulative effect on leaf angle was found when the concentration of colchicines solution was 2.0 mg/mL.
Bauhinia variegatecolchicinemutation breedinggrowth index
NETL
NSTL
万方数据
维普
