In order to study the different effects of germination of Pinus massoniana seeds under different soaking time, the seeds of Masson pine were immersed in water at 21℃ for 12, 24, 48, 72, 96, 120 and 144 h, respectively, then plated in culture dishes. The results showed that: The germination percentage of seeds soaked for 24, 48, 72, 96 and 120 h were higher, but the difference was not significant among the above five soaking treatments. After soaking for 12 h or 144 h, the germination rate was relatively low. Both 12-hour soaking time and 144-hour soaking time were significant different from other five kinds of seed soaking time (P < 0.05). Based on germination energy and germination index of P. massoniana seeds immersed in seven different soaking periods, it can be included that 48 h is the most appropriate soaking period. The fitting degree of the cubic of three germination indexes is the targest by comparing with 10 curves fitting.