Effects of Different Storage Methods on Seed Germination and Seedling Growth of Camellia gauchowensis
This study aimed to investigate the effects of various storage methods on the germination charac-teristics and seedling growth indexes of Camellia gauchowensis,thereby identifying the optimal methods that would provide a scientific basis for its preservation and utilization.The research employed C.gauchowensis seeds as experimental materials,subjecting them to five storage treatments and comparing their germination character-istics and seedling growth metrics.The results showed that(1)There were significant differences(P<0.05)in the germination characteristics among seeds stored under different conditions.The germination rate and germina-tion potential exhibited a declining trend with increased storage duration,except for a marginal difference be-tween 2 and 3 months of storage.The-79℃ultra-low temperature treatment proved most effective,with higher germination rate,germination percentage,and germination potential compared to other treatments,enabling seed viability up to 220 days.Conversely,seeds treated at normal temperature,wax sealing solution A(Mor-pholine fatty acid salts fruit wax),plastic packaging,and wax sealing solution B(RQT-G-4 Fruit preservation and moisturizing brightening agent)were stored for 101 days,the seedling grew slowly,and they were inacti-vated after 162 days of storage.The indexes of wax sealing solution A,plastic packaging,and wax sealing solu-tion B treatment were significantly higher than those of normal temperature treatment.The 4℃low temperature and wax sealing solution C(Orange Freshness Retainer)treatments displayed second-best seed storage proper-ties,extending the shelf life by approximately one month compared to room temperature storage.(2)There were significant variations in shoot length,radicle length,shoot diameter,and radicle diameter among seeds stored using different methods,all diminishing over time.The-79℃ultra-low temperature treatment showed significantly superior indexes compared to other treatments,with the smallest decrease in seedling growth metrics over time,thus proving to be the best storage option.Seeds treated with wax sealing solution C had significantly greater shoot and root lengths,as well as diameters,than those kept at room temperature.The normal tempera-ture treatment demonstrated the poorest effect,indicating its seedlings′ growth ability was most severely affect-ed.Other storage methods,however,mitigated the external environment′s impact on seedling growth and devel-opment to some extent.In summary,the storage methods significantly influenced the seed germination and seed-ling growth of C.gauchowensis.The effect of-79℃ultra-low temperature treatment emerged as the most effi-cacious method,albeit with a high cost.Wax sealing solution C treatment presented the next best alternative,of-fering about a one-month extension in shelf life compared to room temperature storage while being economically viable.This makes it a promising candidate for practical applications in production activities.
Camellia gauchowensisseed storageseed germinationseedling growth index