Identification,Characterization and Analysis of the TIFY Gene Family in Pinus elliottii×P.caribaea
Pinus elliottii×P.caribaea is an important timber and resin production artificial afforestation tree species in southern China due to its high oleoresin yield,straight trunk,and strong resistance to adversity.The TIFY gene family plays a significant role in plant growth and development,in response to abiotic stress,and in the regulation of hormone signal transduction networks.Using bioinformatics techniques,67 TIFY genes were found in the transcriptome of resin tapping treatment in order to examine the function of the TIFY gene family in the process.Their physicochemical properties,gene characteristics,phylogenetic relationships,conserved mo-tifs,conserved domains,and expression patterns were analyzed.Physicochemical analysis revealed that the TIFY proteins of hybrid pine differed in protein length,isoelectric point,and instability index,all of which are hydrophilic proteins.Subcellular localization prediction indicates that the majority(60)of TIFY proteins are lo-cated in the nucleus.Transmembrane structure prediction shows that none of the TIFY proteins contain a trans-membrane structure.Through multi-sequence alignment and systematic evolution,they are divided into four subfamilies,including TIFY(18),ZML(7),PPD(2),and JAZ(40).Conservative motif and conserva-tive domain analysis showed that each TIFY transcription factor has at least one TIFY domain,with different subfamilies exhibiting distinct domain and conservative motif characteristics.Gene expression analysis showed that 56.73%of the PpTIFY gene family members had significant expression changes,mainly in the form of sig-nificantly up-regulated expression,suggesting that the PpTIFY gene family may be involved in transcriptional regulation in response to cutin stress and resin biosynthesis.
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