Primary Study on Tissue Culture Regeneration Technology of Zenia insignis
In order to improve the tissue culture regeneration technology of Zenia insignis,the effects of different contents of NO-3,K+,and Ca2+ in basic medium and different types and concentrations of plant growth regulators on the proliferation and rooting of Z.insignis were investigated by means of micro-cuttings.The re-sults showed as follows:(1)The seeds of Z.insignis were soaked with 75%alcohol for 10 min and 0.1%HgCl2 for 30 min,respectively,and then soaked with sterile water at 85℃for 24 h,which had the best effect,rapid seed germination and seed germination rate can reach 92.16%.(2)The optimal ratio of NO-3,K+ and Ca2+ in RD basic medium was 600 mg/L,360 mg/L and 330mg/L,respectively.The optimal subculture medi-um was RD + 6-BA 0.2 mg/L + NAA 0.01 mg/L,and the proliferation coefficient reached 3.32 at 20 days.(3)NAA had a very significant effect on the rooting and growth of Z.insignis Chun.The optimal rooting medi-um was 1/2MS + 0.5 mg/L NAA+ AC 1.0 g/L,the rooting rate could reach 100%,and the roots were robust and developed,the tissue culture seedlings grew robustly,and the leaves were dark green and stretched.
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