阴香龙脑含量对叶际细菌多样性的影响
The Effect of Cinnamomum burmanni Content on the Diversity of Phyllospheric Microorganism
张宏博 1李兵 2钟泳林 2张谦 2何波祥 2汪迎利 2侯晨 2殷祚云3
作者信息
- 1. 仲恺农业工程学院,广东 广州 510225;广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东 广州 510520
- 2. 广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东 广州 510520
- 3. 仲恺农业工程学院,广东 广州 510225
- 折叠
摘要
为了解龙脑含量的高低对阴香Cinnamomum burmanni叶不同发育阶段叶际细菌群多样性的影响,文章以2 个阴香品系(高龙脑含量—H和低含量—L)的2 个发育时期叶片(嫩叶和成熟叶)为研究材料,利用气相-质谱联用仪检测4 组样本的龙脑含量,利用 16S测序结果比较分析叶际细菌结构和相对丰度的变化.结果表明:(1)相较于嫩叶,2 个品系的成熟叶中龙脑含量均显著增加.(2)4 组样本共检测出259 个操作分类单元,其中H特有操作分类单元的数量最多.(3)基于这些H成熟叶的菌群α多样性指数在4 组样本中最高,高龙脑含量品系的叶片中叶际细菌群落多样性随着叶片成熟而提升,低龙脑含量品系的叶际细菌群落多样性随着叶片成熟变化并不显著.4 组样本之间菌群丰度的差异主要集中在鞘氨醇单胞菌属菌Sphingomonas sp.、短小杆菌属菌Curtobacterium sp.、假单胞菌属菌Pseudomonas sp.、肠杆菌科菌Enterobacteriaceae sp.、类芽孢杆菌属菌Paenibacillus sp.和芽孢杆菌属菌Bacillus sp..研究结果表明阴香叶中龙脑含量的变化影响了叶际细菌菌群结构和丰度,阴香叶片中的龙脑含量越高则叶际细菌菌群多样性随着叶片成熟后越容易发生变化.
Abstract
There are few reports on the effect of borneol content on the biodiversity of phyllospheric micro-organisms in the leaves of borneol-type Cinnamomum burmanni at different developmental stages.Accordingly,in this paper,the leaves of two C.burmanni varieties(high borneol content-H and low borneol content-L)at two developmental stages(tender and mature)were used as the study materials,and the content of borneol was detected in four groups of samples by gas chromatography-mass spectrometry(GC-MS),and the results of 16S sequencing were used to comparatively analyze the changes in the structure and relative abundance of the phyllo-spheric microorganisms.The results showed that(1)the content of borneol in mature leaves of both strains sig-nificantly increased compared to tender leaves.(2)A total of 259 operational taxonomic units(OTUs)were detected in the 4 sets of samples,with the highest number of H-specific OTUs.(3)The α-diversity index of the bacterial community based on these H mature leaves was the highest among the four groups of samples,and the diversity of the phyllospheric microorganisms in the leaves of the high borneol-content varieties increased with leaf maturity,while the diversity of the phyllospheric microorganisms in the low borneol-content lines did not change significantly.The differences in bacterial abundance among the four groups of samples were mainly focused on Sphingomonas sp.,Curtobacterium sp.,Pseudomonas sp.,Enterobacteriaceae sp.,Paenibacillus sp.and Bacillus sp..The research results indicate that changes in the content of borneol in C.burmanni leaves affect the structure and abundance of bacterial communities in the leaf area.The higher the content of borneol in C.burmanni leaves,the easier it is for the diversity of bacterial communities in the leaf area to change as the ma-ture leaves.
关键词
阴香/龙脑/叶际细菌/生物多样性Key words
Cinnamomum burmanni/borneol/interleaf bacteria/biodiversity引用本文复制引用
基金项目
广东省林业科研项目(2023KYXM08)
广东省林业科技创新项目(2022KJCX006)
出版年
2024
NETL
NSTL
万方数据