Models for Determining Active Ingredients Content in Ficus hirta by Using Near Infrared Spectroscopy
By using near infrared reflectance spectroscopy(NIRS),the water content and active ingredi-ents of Ficus hirta collected from Guangdong province were determined,and the corresponding quantitative models were established,and the prediction effect of models was evaluated.The rapid determination of water content and active ingredient content in F.hirta was established.292 samples of F.hirta were collected from five major producing areas of Guangdong province,including Zhanjiang,Maoming,Yunfu,Heyuan and Meizhou.On the basis of high performance liquid chromatography(HPLC),using NIRS combined with par-tial least squares(PLS),NIRS quantitative models were established for the determination of water content,psoralen and bergapten content in the root of F.hirta.The correlation coefficients for water content,psoralen and bergapten content were 0.97,0.91 and 0.83 respectively,and RMSEC were 0.23,0.18,0.03 respec-tively,and RMSECV were 0.27,0.25 and 0.04 respectively,and RMSEP were respectively 0.20,0.20 and 0.03.The NIRS quantitative models of water content were efficient and robust,and the detection accuracy reached general requirements for near infrared rapid detection equipment,which can satisfy the daily needs of detection.The NIRS quantitative models of psoralen content have good effect and good correlation.The effect of the bergapten content model is poor,due to the low content of this index and the relatively concentrated distri-bution gradient.So it is suggested that NIRS can be used as the reference value of bergapten content initial screening in the root of F.hirta.
NETL
NSTL
万方数据
