一株萎缩芽孢杆菌XW-2对松材线虫的杀线活性
The Activity of Bacillus atrophaeus XW-2 Against Bursaphelenchus xylophilus
肖海燕 1黄华毅 2胡铭彪 2龙锦发 2陈刘生 2黄咏槐 2赵丹阳 2扈丽丽2
作者信息
- 1. 韶关市国有林场事务中心,广东 韶关 512000
- 2. 广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东 广州 510520
- 折叠
摘要
为明确萎缩芽孢杆菌Bacillus atrophaeus XW-2 对松材线虫Bursaphelenchus xylophilus的杀线活性,开发松材线虫病生防制剂,研究通过室内浸渍试验,测定了XW-2 菌悬液和发酵上清液对松材线虫的杀线活性.结果表明,XW-2 的菌悬液和发酵上清液原液处理松材线虫 12 h时,松材线虫死亡率分别为100%和91.00%;处理24 h的死亡率均可达100%;随着稀释倍数的增大,线虫死亡率逐渐降低,稀释100 倍时,菌悬液处理松材线虫24 h的线虫死亡率为86.67%,发酵上清液处理的线虫死亡率为 63.00%.因此,XW-2 菌悬液和发酵上清液对松材线虫均具有杀线活性,相同浓度下的菌悬液杀线活性显著(P<0.05)高于发酵上清液.
Abstract
To determine the killing activity of Bacillus atrophaeus XW-2 against the Bursaphelenchus xylo-philus,a biocontrol agent against pine wood nematode disease was developed.The killing activity of XW-2 bacterial suspension and fermentation supernatant against Bursaphelenchus xylophilus was determined by the in-door immersion test.The results showed that when XW-2 bacterial suspension and fermentation supernatant were used to treat pine wood nematodes for 12 hours,the correction rates for pine wood nematodes were 100%and 91.00%,respectively.However,after 24 h of treatment,the corrected mortality rates reached 100%.As the dilution concentration increases,the mortality rate of nematodes gradually decreases.However,after dilu-ting the bacterial suspension 100 times and treating nematodes for 24 hours,the corrected mortality rate of nema-todes is 86.67%.However,after diluting the fermentation supernatant 100 times and treating pine wood nema-todes for 24 hours,the corrected mortality rate of nematodes is 63.00%.Therefore,the XW-2 bacterial sus-pension and fermentation supernatant both had bactericidal activity against pine wood nematodes,and the bacte-ricidal activity of the bacterial suspension at the same concentration was significantly(P<0.05)higher than that of the fermentation supernatant.
关键词
生防制剂/松材线虫/综合防控Key words
biocontrol agents/pine wood nematode/comprehensive prevention and control引用本文复制引用
出版年
2024
NETL
NSTL
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