The Activity of Bacillus atrophaeus XW-2 Against Bursaphelenchus xylophilus
To determine the killing activity of Bacillus atrophaeus XW-2 against the Bursaphelenchus xylo-philus,a biocontrol agent against pine wood nematode disease was developed.The killing activity of XW-2 bacterial suspension and fermentation supernatant against Bursaphelenchus xylophilus was determined by the in-door immersion test.The results showed that when XW-2 bacterial suspension and fermentation supernatant were used to treat pine wood nematodes for 12 hours,the correction rates for pine wood nematodes were 100%and 91.00%,respectively.However,after 24 h of treatment,the corrected mortality rates reached 100%.As the dilution concentration increases,the mortality rate of nematodes gradually decreases.However,after dilu-ting the bacterial suspension 100 times and treating nematodes for 24 hours,the corrected mortality rate of nema-todes is 86.67%.However,after diluting the fermentation supernatant 100 times and treating pine wood nema-todes for 24 hours,the corrected mortality rate of nematodes is 63.00%.Therefore,the XW-2 bacterial sus-pension and fermentation supernatant both had bactericidal activity against pine wood nematodes,and the bacte-ricidal activity of the bacterial suspension at the same concentration was significantly(P<0.05)higher than that of the fermentation supernatant.
biocontrol agentspine wood nematodecomprehensive prevention and control
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