Isolation and Characterisation of Pathogenic Fungi that Lead to Leaf Spot Disease in Cinnamomum burmanni
To increase the biomass of borneol-type Cinnamomum burmanni branches and leaves as well as the yield and quality of distilled borneol essential oil,the morphological characteristics of the pathogen of leaf spot disease in C.burmanni were identified by electron microscopy,and pathogenic fungi of leaf spot disease were isolated and purified from the infected leaves of C.burmanni.These pathogenic fungi were screened using Koch′s Rule,and further identified through morphological characterisation,amplicafication of ITS(Internal transcribed spacer)fragments,DNA sequencing and construction of a phylogenetic tree.The results showed that Colletotrichum gloeosporioides and Colletotrichum siamense are likely pathogenic fungi that lead to leaf spot disease in C.burmanni.
Cinnamomum burmannileaf spot diseasepathogenic fungal identificationITS fragmentsphylogenetic tree
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万方数据
