阴香叶斑病病原真菌的分离与鉴定
Isolation and Characterisation of Pathogenic Fungi that Lead to Leaf Spot Disease in Cinnamomum burmanni
张宏博 1黄华毅 2郭智威 3侯晨 2张谦 2李兵 2汪迎利 2殷祚云4
作者信息
- 1. 仲恺农业工程学院园艺园林学院,广东 广州 510225;广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东 广州 510520
- 2. 广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东 广州 510520
- 3. 广东省龙眼洞林场,广东 广州 510520
- 4. 仲恺农业工程学院园艺园林学院,广东 广州 510225
- 折叠
摘要
为提高龙脑型阴香枝叶的生物量和蒸馏后龙脑精油的产量和质量,研究通过电镜识别阴香叶斑病病原菌的形态学特征,分离、纯化阴香叶病斑中的真菌,利用柯赫氏法则筛选出阴香叶斑病的致病真菌,再经形态学特征观察、ITS(Internal transcribed spacer)片段扩增、DNA测序和系统发育树的构建明确致病真菌的种类.结果表明,胶孢炭疽菌Colletotrichum gloeosporioides和暹罗刺盘孢Colletotrichum siamense可能是阴香叶斑病的病原真菌.
Abstract
To increase the biomass of borneol-type Cinnamomum burmanni branches and leaves as well as the yield and quality of distilled borneol essential oil,the morphological characteristics of the pathogen of leaf spot disease in C.burmanni were identified by electron microscopy,and pathogenic fungi of leaf spot disease were isolated and purified from the infected leaves of C.burmanni.These pathogenic fungi were screened using Koch′s Rule,and further identified through morphological characterisation,amplicafication of ITS(Internal transcribed spacer)fragments,DNA sequencing and construction of a phylogenetic tree.The results showed that Colletotrichum gloeosporioides and Colletotrichum siamense are likely pathogenic fungi that lead to leaf spot disease in C.burmanni.
关键词
阴香/叶斑病/真菌鉴定/ITS片段/系统发育树Key words
Cinnamomum burmanni/leaf spot disease/pathogenic fungal identification/ITS fragments/phylogenetic tree引用本文复制引用
出版年
2024
NETL
NSTL
万方数据