[目的]大红花(Hibiscus rosa-sinensis L。)因花色丰富、观赏价值较高,被广泛应用于华南地区的城市绿化与园林造景中。研究大红花不同花色中花青素种类及含量的特征差异,分析大红花不同品种间代谢物特异性及共同性特征,为深入挖掘品种特性及花色定向育种提供理论和技术支撑。[方法]利用靶向代谢组学技术对白色(W)、红色(R)、黄色(Y)3 种花色大红花花瓣组织中的花青素苷组成和含量进行测定,利用通路富集分析及多元统计学方法对代谢物进行功能注释、鉴定与分析。[结果]大红花W、R与Y花色中共检测出90种代谢物,其中差异代谢物共有 78 种,主要为矢车菊素(41。11%)、飞燕草素(20。00%)、芍药花素(13。33%)等。不同花色大红花间差异代谢物分别为62个(Y vs R)、50个(Y vs W)和65个(R vs W),其中共有差异代谢物22个。综合考虑各个比较组合差异显著性和绝对含量,发现矢车菊素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、矢车菊素-3-O-龙胆二糖苷和芍药花素-3-O-葡萄糖苷含量在红色花中较高;飞燕草素-3-O-(6''-O-酒石酰)葡萄糖苷、原花青素B4 和原花青素B3 含量在黄色花中较高。KEGG代谢通路分析发现差异代谢物在不同花色的组织中显著富集。[结论]矢车菊素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、矢车菊素-3-O-龙胆二糖苷和芍药花素-3-O-葡萄糖苷是大红花呈红色的重要代谢物,原花青素B3 是花色呈黄色的重要代谢物。该结果初步揭示大红花不同花色的代谢物差异,可为大红花花色形成机制和花色改良提供理论参考。
Analysis on Differences in Anthocyanins of Hibiscus rosa-sinensi with Different Flower Colors Based on Targeted Metabolomics
[Objective]Hibiscus rosa-sinensis L.has high ornamental value due to rich flower colors and is widely used in urban greening and landscape architecture in South China.In order to provide theoretical and technical support for in-depth excavation of variety characteristics and flower color directional breeding technology,the characteristic differences of types and contents of anthocyanin in different flower colors were detected,and the specificity and common characteristics of different varieties of H.rosa-sinensis L.were analyzed.[Method]The anthocyanin composition and content of different petal tissues of white(W),red(R)and yellow(Y)flowers were determined by targeted metabolomics.Functional annotation,identification and analysis of metabolites were performed by using pathway enrichment analysis and multivariate statistical methods.[Result]The results showed that a total of 90 metabolites were detected in W,R and Y colors of H.rosa-sinensis L.,among which 78 were differential metabolites,including cyanidin(41.11%),delphinidin(20.00%),peonidin(13.33%),and etc.There were 62(Y vs R),50(Y vs W)and 65(R vs W)differential metabolites among different flower colors,with a total of 22 common differential metabolites.Comprehensively considering the significance and absolute contents of differences in different comparative combinations,it was found thta the cyanidin-3-O-sambubioside-glucoside,pelargonidin-3-O-sophoroside,cyanidin-3-O-gentiobioside and peonidin-3-O-glucoside were relatively high in red flower;the delphinidin-3-O-(6''-O-tartaryl)glucoside,procyanidin B4 and procyanidin B3 were relatively high in yellow flower.KEGG metabolic pathways analysis revealed that differential metabolites were significantly enriched in different flower color tissues.[Conclusion]The cyanidin-3-O-sambubioside-glucoside,pelargonidin-3-O-sophoroside,cyanidin-3-O-gentiobioside and peonidin-3-O-glucoside were important metabolites determining the flower red color,while procyanidin B3 was an important metabolite determining the flower yellow color.This result preliminary revealed the differences in metabolites among different colors of H.rosa-sinensis L.,which could provide theoretical references for the mechanism of color formation and color improvement for H.rosa-sinensis L..