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石橄榄组织培养外植体筛选及培养条件优化

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[目的]建立石橄榄组织培养快繁技术体系。[方法]以石橄榄全株为试材，研究外植体的生长情况，筛选出最佳外植体及培养基，优化石橄榄种子萌发、壮苗增殖等培养条件。[结果]石橄榄组织培养最佳外植体为种子，其根、根茎、假鳞茎均未萌发，并出现长霉和化水的情况，而种子均可萌发。石橄榄种子萌发启动最佳培养基配方为 1/2MS+6-BA 1。5 mg/L+NAA 0。8 mg/L+6-KT 0。1 mg/L，此条件下石橄榄原球茎变绿色。在基础培养基中分别加入香蕉泥(50 g/L)、土豆泥(50 g/L)、椰汁(50 mL/L)进行壮苗有机物筛选发现，添加香蕉泥的培养基中石橄榄整体长势良好、均一，假鳞茎多，株高与叶长均高于培养基添加土豆泥或椰汁的石橄榄，最终选出 50 g/L香蕉泥作为最佳添加有机物加入后续试验的培养基中。石橄榄壮苗优化的最佳培养配方为 1/2MS+50 g/L香蕉泥+6-BA 0。5 mg/L+NA A 0。5 mg/L+6-KT 0。3 mg/L，此条件下石橄榄长势好、株高较高。石橄榄壮苗生根的最佳培养基配方为MS+50 g/L香蕉泥+IBA 0。5 mg/L+NAA 0。5 mg/L，此条件下石橄榄假鳞茎多、气生根多立于苗上下。[结论]建立适合石橄榄种子组织培养快繁技术体系，石橄榄种子最佳萌发启动培养基配方为 1/2MS+6-BA 1。5 mg/L+NAA 0。8 mg/L+6-KT 0。1 mg/L;最佳壮苗优化培养基配方是 1/2MS+50 g/L香蕉泥+6-BA 0。5 mg/L+NAA 0。5 mg/L+6-KT 0。3 mg/L;最佳生根培养基配方是MS+50 g/L香蕉泥+IBA 0。5 mg/L+NAA 0。5 mg/L，为石橄榄的资源保护与利用及其规模化生产奠定基础。
Selection of Explants and Optimization of Culture Conditions for Tissue Culture of Pholidota chinensis Lindl.
[Objective]The study was carried out to establish a technical system suitable for tissue culture and rapid propagation of Pholidota chinensis Lindl.[Method]With the whole plant of P.chinensis Lindl as test material,the growth of explants was studied,the best explant and culture medium were selected,and the culture conditions such as seed germination and seedling proliferation were optimized.[Result]The best explant for tissue culture of P.chinensis Lindl.was the seed,and its roots,rhizomes and pseudobulbs did not germinate,but the seeds could germinate.The optimum culture formula of P.chinensis Lindl.was 1/2MS+6-BA 1.5 mg/L+NAA 0.8 mg/L+6-KT 0.1 mg/L and the protocorm of P.chinensis Lindl.turned green under this condition.The organic matter selection of strong se edlings was conducted by adding banana puree(50 g/L),mashed potato(50 g/L)and coconut water(50 mL/L)to the basal medium,and it was found that the overall growth of P.chinensis Lindl.in the medium with banana puree was good and uniform with many pseudobulbs,and the plant height and leaf length were better than those of the P.chinensis Lindl.with mashed potato or coconut water added to the medium.Finally,50 g/L banana puree was selected as the best organic matter added to the medium in the subsequent experiment.The best culture formula for the optimization of strong seedlings was 1/2MS+50 g/L banana puree+6-BA 0.5 mg/L+NAA 0.5 mg/L+6-KT 0.3 mg/L.Under this condition,the P.chinensis Lindl.grew well and the plant height was better.The best medium formula for rooting of strong seedlings was MS+50 g/L banana puree+IBA 0.5 mg/L+NAA 0.5 mg/L.Under this condition,there were more pseudobulbs and more aerial roots on the seedlings.[Conclusion]To establish a technical system suitable for tissue culture and rapid propagation of P.chinensis Lindl.seeds,the best formula for the germination culture of P.chinensis Lindl.seeds is 1/2MS+6-BA 1.5 mg/L+NAA 0.8 mg/L+6-KT 0.1 mg/L;the optimum culture formula for the optimization of strong seedlings is 1/2MS+50 g/L banana puree+6-BA 0.5 mg/L+NAA 0.5 mg/L+6-KT 0.3 mg/L;the best medium formula for rooting is MS+50 g/L banana puree+IBA 0.5 mg/L+NAA 0.5 mg/L.The study results lay a foundation for the resource conservation and utilization of P.chinensis Lindl.and its large-scale production.

Pholidota chinensis Lindl.tissue cultureculture formulaseed germinationstrong seedlingsrooting

朱华、吴焕、宋巧玲、张淼、陈龙、黄诗萍、梁仁涛、吴文源

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广西中医药大学广西壮瑶药重点实验室/广西中医药大学壮瑶药协同创新中心/广西壮族自治区民族药资源与应用工程研究中心,广西 南宁 530200

石橄榄 组织培养 培养配方 种子萌发 壮苗 生根

广西高校中青年教师科研基础能力提升项目广西一流学科中药学(民族药学)项目广西中医药重点学科壮药学项目广西壮瑶药重点实验室项目

2024KY0310桂教科研[2018]12号GZXK-Z-20-64桂科基字[2014]32号

2024

10.16768/j.issn.1004-874X.2024.08.008

广东农业科学
广东省农业科学院 华南农业大学

广东农业科学

CSTPCD
影响因子：0.556
ISSN：1004-874X
年,卷(期)：2024.51(8)