摘要
[目的]研究氮、磷、碳和铁营养盐对波吉卵囊藻(Oocystis borgei)生长的影响,筛选出最佳营养盐配方,为其高效扩培提供参考.[方法]先利用单因素试验分析不同氮、磷、碳和铁源对波吉卵囊藻生长的影响,获得各自最佳营养盐及最适浓度,再利用正交试验对波吉卵囊藻生长影响较大因素进行优化,得出最佳营养盐配方.[结果]单因素试验结果表明,波吉卵囊藻的最佳氮、磷、碳和铁源及浓度分别是:CO(NH2)2 160 mg/L、C3H7Na2O6P 8 mg/L、NaHCO3 1 g/L、FeCl3 0.2 mg/L;正交试验结果表明,各营养盐对波吉卵囊藻生长影响大小为:磷源＞氮源＞碳源,其中磷源对波吉卵囊藻生长影响最为显著(P<0.05),最佳营养盐配方为:CO(NH2)2 120 mg/L、C3H7Na2O6P 12 mg/L、NaHCO3 2.5 g/L,最优条件下藻细胞密度可达22.07×106 cell/mL,比优化前(1.74×106 cell/mL)提高12倍以上.[结论]确定了波吉卵囊藻最适氮、磷、碳和铁源及其浓度,且通过优化条件得出最佳营养盐配方,显著提高藻细胞密度.
Abstract
[Objective]The effects of nitrogen,phosphorus,carbon and iron nutrients on the growth of Oocystis borgei were explored,and the optimal nutrient formula was selected to provide references for its efficient and large-scale cultivation.[Method]Single factor test was used to analyze the effects of different nitrogen,phosphorus,carbon and iron nutrients on the growth of O.borgei,to obtain the best nutrients and their respective concentrations.Orthogonal tests were conducted to optimize the factors that had a great impact on the the growth of O.borgei.Finally,the optimal nutrient formula was obtained.[Result]The results of the single factor test showed that the optimal sources and concentrations of nitrogen,phosphorus,carbon and iron sources for O.borgei were CO(NH2)2 160 mg/L,C3H7Na2O6P 8 mg/L,NaHCO3 1 g/L,and FeCl3 0.2 mg/L,respectively.The results of the orthogonal tests showed that the effect of each nutrient on the growth of O.borgei ranked as:phosphorus source>nitrogen source>carbon source,and phosphorus source had the most significant effect on the growth of O.borgei(P<0.05).The optimal formula was:CO(NH2)2 120 mg/L,C3H7Na2O6P 12 mg/L and NaHCO3 2.5 g/L.Under the optimal condition,the algal cell density reached 22.07×106 cells/mL,which was 12 times higher than that before optimization(1.74×106 cells/mL).[Conclusion]The optimal nitrogen,phosphorus,carbon,and iron sources and concentrations for O.borgei were determined and the optimal nutrient formula was obtained by optimizing the conditions,which significantly increased the algal cell density.
维普
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